Chinese name: 5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

English name: 5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

Abbreviation: X-Glc

CAS No.: 15548-60-4

Molecular Formula: C14H15BrCINO6

Molecular Weight: 408.63

Specification: 1 g/5 g/25 g (custom packaging supported)

Appearance: off-white crystalline powder

Catalog No.: G01010001

Signal: Blue

Stock: In stock

The application basis of X-Glc lies in its ability to participate in β-D-glucosidase-mediated chromogenic reactions.

When β-D-glucosidase capable of hydrolyzing X-Glc is present in the system, the substrate undergoes enzymatic hydrolysis; under suitable conditions, the intermediate is further oxidized and dimerized to form an insoluble blue chromogenic product.

This process converts the enzymatic reaction into an observable color signal. In microbial chromogenic culture media, R&D personnel can use this reaction characteristic to use β-D-glucosidase activity as a detection indicator in the culture medium identification system.

The blue chromogenic reaction provides an intuitive enzymatic observation basis for microbial identification, and the specific application effect is closely related to the characteristics of the target microorganism and the culture medium system design.

After understanding the chromogenic principle of X-Glc, let us look at the specific applications of this reaction in microbial chromogenic culture media and enzyme activity detection.

1. β-D-Glucosidase Chromogenic Detection and Development of Microbial Identification Culture Media

By utilizing differences in β-D-glucosidase activity among microorganisms and using X-Glc as a chromogenic substrate, it provides a reaction basis for the design of microbial chromogenic culture media. Related applications involve chromogenic detection systems for microorganisms such as Enterococcus, Listeria, Klebsiella, and Enterobacter, and can be used for differential culture medium development, formulation optimization, and multi-indicator chromogenic system research.

2. β-D-Glucosidase Activity Detection and Chromogenic Method Development

By utilizing the reaction characteristic that X-Glc forms a blue chromogenic product after hydrolysis by β-D-glucosidase, it can carry out β-D-glucosidase activity detection, chromogenic substrate evaluation, and related analytical method research, and can be used for enzymatic experiments and chromogenic detection method development.

For chromogenic culture medium development, the substrate being able to produce color is only the starting point of evaluation. What really needs attention is whether this reaction meets the target detection needs and whether it can provide the expected identification information in the complete formulation.

When selecting X-Glc, evaluation can be carried out from the following three aspects.

1. First clarify the detection target: which enzymatic characteristic needs to be identified?

Different chromogenic substrates correspond to different enzymatic indicators. X-Glc is used for β-D-glucosidase chromogenic reactions, so before selection, it should first be confirmed whether the target detection system needs to use this enzyme activity for detection or identification.

At the same time, it is also necessary to understand the enzyme activity performance of target microorganisms and related non-target microorganisms. Only when this indicator can provide effective information for the expected differentiation task does the substrate reaction have corresponding application significance.

2. Then look at the overall formulation: can the blue signal meet the interpretation needs?

In chromogenic culture media, substrates do not work independently. Other components in the culture medium, formulation conditions, and the growth and enzyme activity performance of different microorganisms may all affect the final colony characteristics.

Therefore, when evaluating X-Glc, one should not only observe whether it produces a blue product, but also pay attention to the performance differences between target bacteria and non-target bacteria, and whether the chromogenic reaction can cooperate with other identification indicators.

For culture media using multiple substrates or multiple reaction mechanisms, it is also necessary to examine whether there is interference between different reactions and whether the final colony performance meets the preset interpretation requirements.

3. Finally verify reaction conditions: from substrate reaction to method applicability

Even if the enzymatic positioning of the substrate meets the detection needs, its actual performance still needs to be verified in a specific system. Factors such as substrate concentration, culture medium composition, culture conditions, and interpretation time may all affect the chromogenic results.

Therefore, during formulation development or optimization, evaluation should be carried out around target bacteria, related non-target bacteria, and expected interpretation standards to confirm the performance of the substrate under actual application conditions.

For X-Glc, the clear β-D-glucosidase chromogenic positioning provides direction for substrate screening; its applicability in a specific culture medium needs to be judged through the complete formulation and actual detection conditions.

From substrate selection to culture medium development, the key is not to pursue color development alone, but to match the enzymatic reaction with the target detection needs.

✅ Clear enzymatic positioning, convenient for matching detection needs

X-Glc is a β-D-glucosidase chromogenic substrate suitable for substrate screening and detection method research around this enzyme activity. Clear enzymatic positioning helps R&D personnel select suitable substrates according to target detection indicators.

✅ Intuitive blue chromogenic reaction, convenient for observing enzymatic reaction results

After hydrolysis by β-D-glucosidase, the substrate releases an indolol intermediate, which undergoes oxidative dimerization to form an insoluble blue precipitate; combined with colony color observation, it provides raw material support for chromogenic culture media and enzyme activity assay method development.

✅ Can be used for compatibility evaluation of various R&D systems

X-Glc has public technical materials for chromogenic detection of microorganisms such as Listeria and Enterococcus and for β-D-glucosidase activity research, and can provide reference for microbial testing R&D, culture medium formulation design, and related enzymatic research.

✅ In-stock supply, supporting customized packaging

X-Glc is available in stock, with specifications covering 1 g, 5 g, and 25 g, and supports customized packaging, making it convenient for customers to choose flexibly according to actual R&D usage and meet different experimental scale and procurement needs.