Although different microorganisms may have similar culture characteristics, their enzyme profiles often differ significantly. In the microbial identification process, laboratory personnel often use biochemical indicators such as oxidase, glycosidase, protease, and esterase for auxiliary judgment.

Butyrate esterase is one such enzymatic indicator with identification value. Taking the differentiation of Moraxella catarrhalis and some Neisseria bacteria as an example, Moraxella catarrhalis usually has butyrate esterase activity, while some Neisseria bacteria do not exhibit this feature. Utilizing the difference in enzyme activity reactions between the two can provide auxiliary basis for the identification of related species.

Compared with relying solely on colony morphology, enzymatic reactions can supplement the information provided by colony morphology from a functional level, adding a discrimination dimension to microbial identification, and also providing a basis for the development of biochemical identification systems and related detection methods.

In microbial testing, the reason enzyme activity can become an identification basis is essentially that different microorganisms have different enzymatic characteristics.

For butyrate esterase detection, what laboratory personnel focus on is not the enzyme itself, but how to convert this enzymatic difference into an observable and interpretable detection signal.

In esterase detection systems, a common implementation method is to introduce a chromogenic substrate that can be recognized by the target enzyme and produce a color change. X-Butyrate (5-Bromo-4-chloro-3-inodoxyl butyrate) belongs to this category of functional substrate raw materials.

When butyrate esterase and some related esterase activities are present in the system, the butyrate group in the substrate will be catalyzed and hydrolyzed by the enzyme, releasing the corresponding indole derivative. This intermediate product spontaneously undergoes oxidative coupling (dimerization) in the presence of oxygen, ultimately forming a blue insoluble precipitate.

This process realizes the conversion from "enzyme activity signal" to "color signal", enabling enzymatic reactions that are originally difficult to observe directly to be presented in a visual way.

For chromogenic culture media development, this detection mode can integrate microbial growth and enzymatic identification into the same system. Laboratory personnel can not only observe whether microorganisms grow, but also simultaneously obtain enzyme activity-related information, thereby improving the identification ability and result readability of the culture medium.

Chinese name: 5-Bromo-4-chloro-3-indolyl butyrate

English name: 5-Bromo-4-chloro-3-indoxyl butyrate

Abbreviation: X-Butyrate

CAS No.: 129541-43-1

Molecular Formula: C12H11BrClNO2

Molecular Weight: 316.58

Specification: 1 g / 5 g / 25 g (custom packaging supported)

Appearance: off-white crystalline powder

Catalog No.: G02030001

Signal: Blue

Stock: In stock

1. Development of Microbial Biochemical Identification Systems

Used for the development of butyrate esterase reaction modules, providing enzymatic identification indicators for microbial biochemical identification systems. Some microorganisms have characteristic butyrate esterase activity, which can be used as a basis for species differentiation and auxiliary identification. X-Butyrate can be used in the R&D of related identification reagents, identification cards, and automated identification systems.

2. Development of Chromogenic Culture Media and Differential Culture Media

Used for the formulation development of chromogenic culture media and differential culture media. By introducing enzymatic reaction modules, it adds an additional identification dimension to the culture medium. R&D personnel can combine the biochemical characteristics of target bacteria and use it together with functional raw materials such as glycosidase substrates and aminopeptidase substrates to construct a culture media system with differentiated recognition capability.

3. Research on Microbial Esterase Activity

As a typical C4 esterase substrate, it can be used for the activity analysis of butyrate esterase and related carboxylesterases, providing an experimental tool for enzymatic research, substrate screening, and detection method development. It is suitable for research on microbial enzymatic characteristics and evaluation of new detection systems.

✅ Butyrate esterase detection substrate, clear application path

X-Butyrate is a classic C4 esterase chromogenic substrate and can be used for the detection of butyrate esterase and related carboxylesterase activity. Butyrate esterase reactions have been applied in some microbial biochemical identification systems, providing an enzymatic basis for species differentiation and auxiliary identification. The application direction of X-Butyrate chromogenic substrate is clear, the technical route is mature, and it can be directly used for the development of related detection systems.

✅ Suitable for dual-platform development of culture media and identification products

X-Butyrate can be used both for the development of microbial biochemical identification systems and as a functional substrate for chromogenic culture media design. R&D personnel can, according to the enzymatic characteristics of target bacteria, combine it with functional raw materials such as glycosidase substrates and aminopeptidase substrates to construct multi-indicator detection systems, providing more design space for culture media optimization and product innovation.

✅ Suitable for esterase substrate screening and method development

As a typical C4 esterase substrate, X-Butyrate can be used not only for the development of existing detection systems, but also for enzymatic research, substrate evaluation, and establishment of new detection methods. For R&D teams carrying out esterase-related projects, it can serve as an important reference tool for substrate screening and method validation.

✅ Domestic stock supply, smoother connection between R&D and industrialization

Hepattack Bioscience & Technology has long focused on the development and supply of core raw materials for microbial testing, and can provide X-Butyrate stock products and customized packaging services. Specifications cover 1 g, 5 g, and 25 g, meeting the needs of R&D validation, pilot optimization, and subsequent product development, providing customers with more flexible procurement and supply solutions.