Chinese Name: DRAQ5 Dye

English Name: DRAQ5 Dye

Abbreviation: DRAQ5 Dye

CAS Number: 252903-95-0

Molecular Formula: C22H28N4O4

Molecular Weight: 412.49

Specifications: 1 mg / 5 mg / 10 mg (Custom packaging supported)

Form: Lyophilized powder

Product Number: G10040025

Stock: In stock

Color: Red fluorescence (Far red)

DRAQ5 dye is a high-affinity anthraquinone-based fluorescent probe that freely penetrates cell and nuclear membranes and specifically binds to DNA. Upon far-red excitation, the dye emits a stable red fluorescence signal. This characteristic makes it an ideal choice for cell proliferation studies and DNA content analysis, and compared to traditional UV-excited dyes, it effectively reduces background interference and improves experimental accuracy.

• Cell Labeling: Rapidly labels DNA in live or fixed cells, widely used in cell proliferation studies.

• Flow Cytometry Analysis: Quantitatively assesses cell cycle status via DNA content, helping to accurately analyze cell proliferation.

• Fluorescence Microscopy: Utilizes far-red properties to reduce background interference and achieve high-resolution cellular images.

• Immunocytochemistry: Used in combination with other fluorescent probes for multicolor imaging, enhancing data richness.

✅ Easy to Operate: No washing step for live-cell experiments, simplifying the procedure, suitable for automated platforms, improving experimental efficiency.

✅ High Affinity: High affinity for double-stranded DNA, ensuring stable and reliable labeling.

✅ Far-Red Property: Far-red excitation reduces background interference, suitable for multicolor imaging, improving image clarity.

✅ In Stock: Direct shipment, no waiting for import lead times, ensuring continuity and efficiency of experiments.

Q1: What experiments is DRAQ5 dye suitable for?

A: DRAQ5 dye is widely used in cell proliferation studies, flow cytometry, fluorescence microscopy, and immunocytochemistry. It effectively labels DNA and is suitable for cell cycle analysis, DNA quantification, and multicolor co-staining experiments, especially those requiring high sensitivity and low background interference.

Q2: What is the color development principle of DRAQ5 dye?

A: DRAQ5 dye specifically binds to double-stranded DNA and rapidly penetrates cell and nuclear membranes. Upon far-red excitation, it emits stable red fluorescence (emission peak around 697 nm), significantly reducing background interference. The fluorescence signal intensity is linearly related to DNA content, making it suitable for DNA quantification and cell cycle analysis.

Q3: Is this dye compatible with other fluorescent probes?

A: Yes, DRAQ5 dye has far-red excitation properties, making it compatible with other commonly used fluorescent probes (such as FITC, PE, Cy5), especially suitable for multicolor imaging and multiplex detection experiments. Its far-red properties minimize spectral overlap with other dyes, ensuring signal clarity and experimental accuracy.

Q4: What are the spectral properties of DRAQ5 dye? Which excitation/detection channels are suitable?

A: DRAQ5 has an excitation wavelength range of 633~647 nm and an emission peak around 697 nm. Its far-red spectral properties effectively avoid interference from common visible light fluorescent dyes, making it suitable for excitation with far-red lasers (e.g., 633 nm) and compatible with far-red filters, particularly suitable for flow cytometry, fluorescence microscopy, and high-throughput screening.

Q5: What specific advantages does DRAQ5 have over traditional DNA dyes (such as PI or Hoechst)?

A: Compared to traditional DNA dyes, DRAQ5 has higher affinity and sensitivity, and does not require UV excitation (like Hoechst) or ethanol treatment (like PI). Additionally, DRAQ5 effectively reduces background noise and is compatible with other fluorescent dyes, suitable for multicolor experimental designs, providing clearer and more stable signals.

Q6: Is DRAQ5 suitable for automated high-throughput platforms?

A: Yes, due to its lysis/wash-free characteristics, DRAQ5 dye is very suitable for high-throughput automated platforms. It simplifies sample processing steps, rapidly achieves DNA staining, and supports large-scale cell proliferation analysis and cell cycle analysis, greatly improving experimental efficiency.

Q7: What is the shelf life and stability of DRAQ5 dye?

A: DRAQ5 dye has high stability in lyophilized powder form and can be stored for a long time at -20°C. Once dissolved, its fluorescence signal is very stable, suitable for long-term experimental use. Its staining process is not affected by long-term storage, making it very reliable for batch experiments.