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New In-Stock Product | 4MβNA Coagulase Peptide Substrate: Lighting Up Signals for Coagulase/Thrombin Detection

2026/4/10 14:20:38 Browse volume(16)
Coagulase Substrates

4MβNA Peptide Substrates


In scenarios such as microbiology laboratories, coagulation function testing, and protease drug development, the requirements for detecting coagulase activity vary: rapid screening pursues simplicity and efficiency, while precise verification demands high specificity and low background interference.

To this end, Hepattack Bioscience & Technology has launched three peptide substrates based on 4MβNA. Through sequence design and background signal control, they can be recognized by the target enzyme and generate quantifiable fluorescent signals, thereby lowering the technical threshold for detecting trace coagulase activity to some extent.

I. Product List

1. Z-Gly-Pro-Arg-4MβNA

Z-Gly-Pro-Arg-4MβNA 66647-41-4 Structural Formula

Chinese Name: Z-Gly-Pro-Arg-4MβNA acetate

English Name: Z-Gly-Pro-Arg 4-methoxy-β-naphthylamide

Abbreviation: Z-Gly-Pro-Arg-4MβNA

CAS Number: 66647-41-4

Molecular Formula: C32H39N7O6

Molecular Weight: 617.71

Specifications: 25 mg/vial (100 mg/vial, 1 g/vial, custom packaging supported)

Form: Lyophilized powder

Product Number: G12020001

Stock: In stock


2. H-Gly-Pro-Arg-4MβNA ∙ 2HCl

H-Gly-Pro-Arg-4MβNA ∙ 2HCl 74503-74-5 Structural Formula

Chinese Name:Glycyl-prolyl-arginyl-4-methoxy-β-naphthylamide dihydrochloride

English Name: H-Gly-Pro-Arg-4MβNA ∙ 2HCl

Abbreviation: H-Gly-Pro-Arg-4MβNA ∙ 2HCl

CAS Number: 74503-74-5

Molecular Formula: C24H35Cl2N7O4

Molecular Weight: 556.49

Specifications: 25 mg/vial (100 mg/vial, 1 g/vial, custom packaging supported)

Form: Lyophilized powder

Product Number: G04020003

Stock: In stock


3. H-Gly-Arg-4MβNA ∙ 2HCl

H-Gly-Arg-4MβNA ∙ 2HCl 100940-56-5 Structural Formula

Chinese Name: Glycyl-arginyl-4-methoxy-β-naphthylamide dihydrochloride

English Name: Gly-Arg 4-methoxy-β-naphthylamide dihydrochloride

Abbreviation: H-Gly-Arg-4MβNA ∙ 2HCl

CAS Number: 100940-56-5

Molecular Formula: C19H28Cl2N6O3

Molecular Weight: 495.37

Specifications: 25 mg/vial (100 mg/vial, 1 g/vial, custom packaging supported)

Form: Lyophilized powder

Product Number: G04030041

Stock: In stock


II. Working Principle

This class of substrates is constructed as arylamide peptide substrates by forming an amide bond between the C-terminus of the X-Arg peptide residue and the 4-methoxy-β-naphthylamine (4MβNA) fluorescent reporter group. In the intact state, the fluorescence of 4MβNA is quenched, thus no fluorescence signal is released.

Taking coagulase detection as an example, signal release occurs in three steps:

1. Recognition and Cleavage: Coagulase specifically recognizes the arylamide peptide bond in the substrate, catalyzes the hydrolysis of the peptide bond, and releases 4MβNA from the peptide chain.

2. Fluorescence Activation: Free 4MβNA emits strong fluorescence (at ~410 nm) upon excitation at ~340 nm. The higher the enzyme activity, the more 4MβNA is released per unit time, and the faster the fluorescence intensity increases.

3. Quantitative Interpretation: By continuously monitoring the rate of change in fluorescence intensity using a fluorescence microplate reader or fluorescence spectrophotometer, the coagulase activity unit (U/mL) can be accurately calculated, achieving a seamless transition from "microsample → minute-level readout → objective data".


III. Application Scenarios

• Coagulation Research: Used for accurate detection of coagulase activity, helping to reveal blood coagulation mechanisms and related diseases;

• Vaginal Infection Typing: Bacterial coagulase positivity can serve as an important indicator suggesting Staphylococcus aureus infection in vaginal infection typing;

• Dipeptidyl Peptidase I (DPP-I) Analysis: Also detects dipeptidyl peptidase I, applicable for studying lysosomal function in leukemia cell lines;


IV. Core Advantages

✅ High Sensitivity
Through changes in fluorescence signal, it can accurately capture coagulase activity changes, providing highly sensitive detection results, especially suitable for low-concentration samples.

✅ Easy to Operate
Suitable for standard fluorescence spectrophotometers or microplate readers, with a simple procedure to obtain accurate experimental results quickly.

✅ High Reliability
Precise molecular design ensures stability of each reaction, avoids background noise interference, and ensures data accuracy and reproducibility.

✅ Quality Assurance
Hepattack Bioscience & Technology offers in-stock supply and supports custom packaging in various sizes to meet the needs of different scales of research.



Extended Services

Change begins with choosing a stable and reliable fluorescent substrate for coagulase detection. We sincerely invite you to experience the excellent performance of the new domestic force.

For more peptide substrates for enzyme activity detection or custom packaging services, please call +86-13302967066!

Hepattack Bioscience & Technology is dedicated to providing reliable domestic alternative solutions for gene sequencing, molecular diagnostics, and microbial detection.

For related products such as blood cell dyes and buffer salts, you can view the complete product line and technical specifications on [MedSun Biotechnology Website].


In Vitro Molecular Diagnostics | Gene Sequencing | Microbial Detection



END


Shenzhen Hepattack Bioscience & Technology Co. Ltd.

Founded in 2015, it is a high-tech enterprise focusing on microbial testing、 gene sequencing and in vitro molecular diagnostics in the R&D, production, and sales of biochemical reagent raw materials. Committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, the product portfolio covers more than a dozen categories and nearly a thousand innovative products. Upholding the core philosophy of “customer first, integrity and professionalism”, we serve with dedication to meet customers’ diverse needs in research and testing.

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