Chinese Name: Cyanine 3 dideoxyadenosine triphosphate solution

English Name: Cyanine 3 dideoxyadenosine triphosphate

Abbreviation: Cy3-ddATP

CAS Number: NA

Molecular Formula: C45H56N7O18P3S2 (free acid)

Molecular Weight: 1140.01 (free acid)

Specifications: 25 ul / 100 ul / 1 ml (Custom packaging supported)

Form: 10 mM aqueous solution (pink)

Spectrum: Ex 550 nm / Em 570 nm

Purity: ≥ 98% (HPLC)

Product Number: G09010025

Stock: In stock

The function of Cy3-ddATP can be precisely summarized by three actions: recognition, termination, luminescence.

(1) Structure: A "Modified" Adenylate

Cy3-ddATP uses ddATP (dideoxyadenosine triphosphate) as its molecular backbone. Compared to natural intracellular dATP, the 3'-hydroxyl group on its ribose is removed, thereby losing the ability for chain extension. Simultaneously, a Cy3 cyanine fluorophore is covalently conjugated to the nucleotide structure via a flexible linker. The triphosphate tail is fully retained — this is the key that DNA polymerase recognizes.

(2) Event: The moment of incorporation, the chain terminates

During DNA synthesis, polymerase treats Cy3-ddATP as a substitute for dATP, incorporating it into the growing strand opposite thymine (T) on the template strand. However, due to the absence of the 3'-OH, the next nucleotide cannot form a phosphodiester bond with it — chain extension terminates irreversibly at this point.

(3) Signal: Cy3 lights up, marking the position of "A"

Each DNA fragment terminated at an A site has a Cy3 fluorophore attached to its 3' end. After separation by capillary electrophoresis according to fragment length, a laser excites at 550 nm, and the detector captures the characteristic fluorescence at 570 nm — in a four-color sequencing system, this pink-orange peak is the identifier for adenine.

• Sanger Sequencing

Used to construct fluorescent chain termination systems, enabling base-specific termination and signal readout, improving sequencing peak clarity and base-calling accuracy;

• IVD Sequencing Reagent Development

As a key fluorescent terminator raw material, used in in vitro diagnostic kit development to enhance batch-to-batch consistency and detection stability;

• DNA 3' End Fluorescent Labeling

Introducing a single Cy3 signal at the DNA chain terminus for fragment detection, probe construction, and nucleic acid quantification analysis;

✅ Precise Termination

Based on the natural lack of the 3'-OH group in the dideoxy structure, deterministic chain termination during DNA polymerization is achieved, preventing extension trailing issues from the source and ensuring clear, interpretable termination sites;

✅ Stable Output

Utilizes the Cy3 fluorophore, which offers good photostability, maintaining stable signal output under long detection periods or multiple scanning cycles;

✅ Clean Signal

HPLC purity ≥ 98%. The production process effectively removes free fluorescent dyes and unmodified nucleotides through efficient purification techniques, ensuring extremely low background absorption in experiments and significantly enhancing detection sensitivity;

✅ Strong System Compatibility

Provided as a 10 mM standard solution with stable pH, it can be directly used with mainstream DNA polymerase systems, reducing pre-processing steps and improving experimental reproducibility;