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New In-Stock Product | L-Tyrosyl-p-nitroanilide (H-Tyr-pNA, CAS: 52551-07-2): A Classic Chromogenic Substrate for Serine Proteases

2026/2/7 11:16:58 Browse volume(73)
Peptidase Chromogenic Substrate

H-Tyr-pNA 


In fields such as basic enzymology research, protease inhibitor drug development, and in vitro diagnostic reagent R&D, precise quantification of serine protease activity – particularly trypsin and chymotrypsin – is a core aspect of many research projects.

L-Tyrosine 4-Nitroanilide (H-Tyr-pNA) is a mature, classic chromogenic substrate solution designed specifically for serine protease activity detection. With its three core advantages of clear reaction mechanism, stable signal output, and economical detection cost, it has become the benchmark choice for many laboratories conducting enzyme kinetic studies and high-throughput screening.

I. Product Overview

L-Tyrosine 4-Nitroanilide (H-Tyr-pNA) 52551-07-2 Structure Formula

Chinese Name: L-Tyrosine 4-Nitroanilide

English Name: L-Tyrosine 4-Nitroanilide

Abbreviation: H-Tyr-pNA

CAS Number: 52551-07-2

Molecular Formula: C15H15N3O4

Molecular Weight: 301.30

Specification: 1 g / 5 g / 25 g (custom packaging supported)

Form: Lyophilized powder

Product Number: G04040014

Color Development: Yellow

Stock: In stock

The core structure of H-Tyr-pNA consists of L-tyrosine (Tyr) and p-nitroaniline (pNA) connected via an amide bond.

When serine proteases such as trypsin and chymotrypsin undergo specific hydrolysis of the substrate, the substrate is cleaved and releases free p-nitroaniline (pNA).

pNA has a significant characteristic absorption peak at a wavelength of 405 nm. By directly reading the absorbance change using a microplate reader or spectrophotometer, quantitative calculation of protease activity or reaction rate can be achieved.



II. Application Scenarios

• Enzymology Research: Used for in vitro determination of serine protease activity such as trypsin and chymotrypsin.

• Drug Screening: Used as a substrate for high-throughput screening of protease inhibitors or activators.

• Diagnostic Reagent Development: Used as a raw material for developing enzyme activity-based in vitro diagnostic kits.

III. Supply Assurance

We deeply understand the high demands of scientific exploration and industrial R&D for delivery cycle, batch consistency, and data stability. Hepattack Bioscience & Technology provides:

• Multi-Specification Stock: Covers common packaging from 1 g (trial) to 25 g (pilot), with ample stock reserves for faster delivery.

• High-Purity Raw Material: The H-Tyr-pNA provided by Hepattack Bioscience & Technology achieves HPLC purity ≥ 98%, with strict control of residual free pNA, ensuring extremely low background absorption and more reliable data.

• Flexible Customization: For industrial clients, we provide gram-level custom synthesis and packaging services, fully supporting your project from R&D to production.


Extended Services

Change begins with choosing a stable and reliable substrate. We sincerely invite you to personally experience the excellent performance of domestic new strength.

For more chromogenic substrates or custom packaging services, please call +86 13302967066!

Hepattack Bioscience & Technology focuses on providing reliable domestic alternative solutions for gene sequencing, molecular diagnostics, and microbial detection.

For related products such as blood cell dyes and buffer salts, you can view the complete product line and technical specifications on the 【MedSun Biotechnology Website】.


In Vitro Molecular Diagnostics | Gene Sequencing | Microbial Detection



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Shenzhen Hepattack Bioscience & Technology Co. Ltd.

Founded in 2015, it is a high-tech enterprise focusing on microbial testing gene sequencing and in vitro molecular diagnostics in the R&D, production, and sales of biochemical reagent raw materials. Committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, the product portfolio covers more than a dozen categories and nearly a thousand innovative products. Upholding the core philosophy of “customer first, integrity and professionalism”, we serve with dedication to meet customers’ diverse needs in research and testing.

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