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New In-Stock Product | Classic Chromogenic Substrate for Serine Proteases: Z-Gly-Pro-Arg-pNA Acetate (CAS: 66648-35-9)

2026/2/6 10:01:00 Browse volume(65)
   
Peptidase Chromogenic Substrate

Z-Gly-Pro-Arg-pNA Acetate


In research on blood coagulation mechanisms and the development of coagulation-related in vitro diagnostic reagents, precise quantitative analysis of serine protease activity – particularly the core enzyme "Thrombin" – is crucial. Traditional detection methods often face issues of insufficient specificity or limited sensitivity.

Z-Gly-Pro-Arg-pNA Acetate, with its specific tripeptide sequence (Gly-Pro-Arg), can be recognized and cleaved by serine proteases with trypsin-like specificity, such as thrombin, making it one of the commonly used chromogenic substrates for constructing colorimetric thrombin activity detection and enzyme kinetic analysis systems.

I. Product Overview

Z-Gly-Pro-Arg-pNA Acetate 66648-35-9 structure formula

Chinese Name: Z-Gly-Pro-Arg-pNA Acetate

English Name: Z-Gly-Pro-Arg-pNA Acetate

Abbreviation: Z-Gly-Pro-Arg-pNA Acetate

CAS Number: 66648-35-9

Molecular Formula: C29H38N8O9

Molecular Weight: 642.67

Specification: 100 mg / 500 mg / 1 g (custom packaging supported)

Form: Lyophilized powder

Product Number: G04020004

Color Development: Yellow

Stock: In stock



II. Core Advantages

✅ Clear reaction mechanism, convenient detection

The structural design of this product mimics the natural substrate cleavage site of thrombin. In the experimental system, thrombin specifically hydrolyzes the amide bond between arginine (Arg) and p-nitroaniline (pNA). The released free pNA (p-nitroaniline) has a distinct characteristic absorption peak at 405 nm. Researchers only need to monitor the change in OD value using a microplate reader or spectrophotometer to accurately calculate the enzyme activity units.

✅ Acetate form: Better solubility and stability

Unlike the common hydrochloride form on the market, our product is provided in the Acetate form. This form is convenient for storage and use. It is easy to prepare in common experimental solvents and compatible with various buffer systems. Other salt forms, such as trifluoroacetate or hydrochloride, can also be provided upon customer request.

✅ High purity control, reducing background interference

For peptide substrates, residual free pNA is a key factor affecting the sensitivity of low-concentration enzyme activity detection. Hepattack Bioscience & Technology's Z-Gly-Pro-Arg-pNA Acetate undergoes strict HPLC purification, with strict control of residual free pNA, reducing background absorption. High batch-to-batch consistency ensures high repeatability of experimental data.



III. Application Scenarios

· Thrombin Activity Assay: For basic scientific research on the coagulation cascade.

· Diagnostic Reagent Raw Material: Used in the development and quality control of coagulation-related detection reagents (e.g., thrombin activity/inhibition system evaluation, etc.).

· Trypsin-like Protease Activity Analysis: Can be used for activity analysis of some trypsin-like proteases such as trypsin, plasmin, etc.

IV. Supply Assurance

We understand the high demand for timeliness in scientific exploration and industrial R&D. Hepattack Bioscience & Technology provides:

In-stock direct shipment: We maintain sufficient in-stock inventory for commonly used specifications from 100 mg (trial) to 500 mg (pilot).

Flexible customization: For industrial clients, we provide gram-level custom synthesis and packaging services, fully supporting your project from R&D to production.




Extended Services

Change begins with choosing a stable and reliable substrate. We sincerely invite you to personally experience the excellent performance of domestic new strength.

For more chromogenic substrates or custom packaging services, please call +86 13302967066!

Hepattack Bioscience & Technology focuses on providing reliable domestic alternative solutions for gene sequencing, molecular diagnostics, and microbial detection.

For related products such as blood cell dyes and buffer salts, you can view the complete product line and technical specifications on the 【MedSun Biotechnology Website】.


In Vitro Molecular Diagnostics | Gene Sequencing | Microbial Detection
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Shenzhen Hepattack Bioscience & Technology Co. Ltd.

Founded in 2015, it is a high-tech enterprise focusing on microbial testing gene sequencing and in vitro molecular diagnostics in the R&D, production, and sales of biochemical reagent raw materials. Committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, the product portfolio covers more than a dozen categories and nearly a thousand innovative products. Upholding the core philosophy of “customer first, integrity and professionalism”, we serve with dedication to meet customers’ diverse needs in research and testing.

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