β-L-fucosidase is an exo-glycosidase that hydrolytically cleaves β-L-fucose residues from the non-reducing ends of glycoconjugates.

In plant physiology, it participates in the metabolism of xyloglucan, affecting cell wall loosening and fruit softening. In microbiology, β-L-fucosidase is involved in bacterial degradation of host glycans and is one of the important components of the glycosidase system. Differing in substrate specificity and physiological function from the common clinical serum marker for liver cancer, α-L-fucosidase (AFU), β-L-fucosidase is a key tool enzyme for studying glycosylation modification, host-microbe interactions, and plant physiology.

· Microbial Functional Characterization: Differentiating the sugar metabolism capabilities of bacterial strains.

· Glycan Chain and Glycosylation Research: Involved in the regulation of fucose modification.

· Lysosome-Related Enzymology Studies: Used for enzyme activity evaluation in cells or model organisms.

· Enzyme Profiling and Screening: Completing the functional map of glycosidases.

β-L-fucosidase specifically hydrolyzes β-L-fucosidic bonds. Introducing a β-L-fucoside substrate carrying a signal label into the reaction system allows the enzymatic cleavage process to simultaneously release a detectable signal molecule.

Depending on the form of the signal label, the reaction result can manifest as color precipitation, colorimetric change, or fluorescence enhancement. As the reaction progresses, the signal intensity correlates with the enzyme activity level, enabling visual interpretation and quantitative analysis of β-L-fucosidase activity.

β-L-fucosidase substrates with different signal labels can transform the same enzyme reaction into colorimetric, chromogenic, or fluorescent readouts, meeting multi-level needs from qualitative judgment to quantitative analysis and high-sensitivity detection.

This design concept of "substrate as signal" endows β-L-fucosidase activity detection with the following advantages:

✅ High Specificity: Relies on β-L conformation recognition, reducing non-specific hydrolysis background.

✅ Visual Signal: Reaction results are visible, facilitating interpretation.

✅ Good Reproducibility: Stable signal output, suitable for comparative analysis.

✅ High Controllability: Applicable to different experimental conditions and detection platforms.

Chinese Name: 5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside

English Name: 5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside

Abbreviation: X-β-L-Fuc

CAS Number: 125328-84-9

Molecular Formula: C14H15BrClNO5

Molecular Weight: 392.63

Specification: 25 mg / 100 mg / 250 mg (custom packaging supported)

Form: Lyophilized powder

Product Number: G01010016

Stock: In stock

Color Development: Blue

Application: Chromogenic substrate (plate/tissue localization)

Chinese Name: 4-Nitrophenyl β-L-fucopyranoside

English Name: 4-Nitrophenyl β-L-fucopyranoside

Abbreviation: PNP-β-L-Fuc

CAS Number: 22153-71-5

Molecular Formula: C12H15NO7

Molecular Weight: 285.25

Specification: 25 mg / 100 mg / 250 mg (custom packaging supported)

Form: Lyophilized powder

Product Number: G01060057

Stock: In stock

Color Development: Yellow

Application: Colorimetric substrate (enzyme activity quantification)

Chinese Name: 4-Methylumbelliferyl β-L-fucopyranoside

English Name: 4-Methylumbelliferyl β-L-fucopyranoside

Abbreviation: 4MU-β-L-Fucoside

CAS Number: 72601-82-2

Molecular Formula: C16H18O7

Molecular Weight: 322.31

Specification: 25 mg / 100 mg / 250 mg (custom packaging supported)

Form: Lyophilized powder

Product Number: G01070021

Stock: In stock

Color Development: Blue fluorescence

Application: Fluorescent substrate (high sensitivity detection)